We narrowed to 6,650 results for: tac

1. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-4T1-USP11

   Plasmid

   #78849

   Purpose

   To purify GST-tagged recombinant USP11 protein

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

   Insert

   USP11 (USP11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-parkin WT

   Plasmid

   #45969

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HAI

   Plasmid

   #71316

   Purpose

   Inducible expression of human arginase I in E. coli

   Depositor

   David Christianson

   Article

   DiCostanzo et al Proc Natl Acad Sci U S A. 2005 Sep 13;10

   Insert

   human arginase I (ARG1 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGex-4T-2_GABARAP

   Plasmid

   #73948

   Purpose

   For recombinant expression of human GABARAP in E.coli

   Depositor

   Dieter Willbold

   Article

   Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGex-6p2_FANCM

   Plasmid

   #221379

   Purpose

   Expresses FANCM in bacterial cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Fanconi anemia group M protein (FANCM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. polyPRM5

   Plasmid

   #175232

   Purpose

   Bacterial expression and purification, mixed with polySH3 to form condensates

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   ABL1 (ABL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGex-6p2_ATRX

   Plasmid

   #221377

   Purpose

   Expresses ATRX in bacterial cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Transcriptional regulator ATRX (ATRX Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T1-Rac1 G15A

   Plasmid

   #69355

   Purpose

   expression of nucleotide free Rac1 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-6p-hDcp2

    Plasmid

    #72216

    Purpose

    bacterial expression of GST-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F146Y and E198K (please see depositor's comm…

    Promoter

    tac

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. HisMBP CFlm25

    Plasmid

    #78458

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie

    Article

    Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

    Insert

    Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. GST-GAR

    Plasmid

    #34697

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-145 of human fibrillarin with K2E and …

    Promoter

    tac

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

13. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-parkin W403A

    Plasmid

    #45974

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin W403A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-parkin C431S

    Plasmid

    #45976

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin C431S (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1‐Afu‐rnhB D101N

    Plasmid

    #108695

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D101N catalytic site mutation

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX4T2-Snf1-cat

    Plasmid

    #52683

    Purpose

    expression of the Snf1 kinase domain in bacteria

    Depositor

    Benjamin Turk

    Article

    Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

    Insert

    SNF1 (SNF1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes kinase domain only, aa 41-315

    Promoter

    tac

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-parkin F463A

    Plasmid

    #45975

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin F463A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AEB-No-aptamer-controlluciferase

    Plasmid

    #63853

    Purpose

    This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #67608

    Purpose

    bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    catalytic mutant LDIG(182-185)AAAA

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only