We narrowed to 80,162 results for: ELL

1. pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

   Plasmid

   #187358

   Purpose

   Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   HPC-4 antibody heavy chain, and 6xHIS tag
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. DCT.VV.Orf170

   Plasmid

   #12858

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf170
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Dual U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. FUW-ubiquitin-SV40-GFP

   Plasmid

   #65445

   Purpose

   Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   SV40-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBQ and SV40

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

   Plasmid

   #61411

   Purpose

   Expresses human OTUD3 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 276-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNAIIIB cdc42 QL

   Plasmid

   #61636

   Purpose

   mammalian expression of cdc42 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Cdc42 (CDC42 Human)
   

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

9. HsCLYBL-FLAG

   Plasmid

   #111290

   Purpose

   mammalian expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   human CLYBL (CLYBL Human)
   

   Tags

   linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HsCLYBL(D320A)-FLAG

    Plasmid

    #111291

    Purpose

    mammalian expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL D320A (CLYBL Human)
    

    Tags

    linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

    Expression

    Mammalian

    Mutation

    D320A

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. VanGlow-GL

    Plasmid

    #83342

    Purpose

    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    GFP::Luciferase(nls)

    Expression

    Insect

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CB6-GFP-TAOK2 (K57A)

    Plasmid

    #197114

    Purpose

    Expression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

    Insert

    TAOK2 (K57A) (TAOK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    CMV

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-Regnase-1 WT

    Plasmid

    #222653

    Purpose

    Mammalian expression vector to express mouse Regnase-1 WT

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KTK_354

    Plasmid

    #180525

    Purpose

    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-GFP-PQRv3-RFP

    Plasmid

    #73951

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP (superfolder GFP)
    RFP
    

    Tags

    A residual PQR peptide wii be left at the C-termi…

    Expression

    Mammalian

    Promoter

    Chicken beta actin and Chicken beta actin (shared…

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

19. psiCHECK-2-TGFBR2-wt_3'UTR

    Plasmid

    #31882

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    TGFBR2 wild-type 3'UTR (TGFBR2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pCXLE-hS + eGFP

    Plasmid

    #74945

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Sox2 (SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only