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TagBFP2-PIP4K2B

Plasmid

#202728

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (PIP4K2B Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKG3560_pHarbor-Rogi2_backbone

Plasmid

#239705

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for Bxb1-mediated integration in the Rogi2 landing pad line

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLA230

Plasmid

#11724

Depositor

Lawrence Loeb

Article

Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

Insert

b-lactamase

Expression

Bacterial

Mutation

GAA for Glu26 changed to TAA (premature stop codo…

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_B0000

Plasmid

#91058

Purpose

Module B, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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Rluc8-NbV5

Plasmid

#201479

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-Rluc8

Tags

Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAFNF-GFP

Plasmid

#13772

Purpose

Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFP

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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dCas9-5xGCN4-P2A-BFP

Plasmid

#184438

Purpose

dCas9 fused with 5 copies of GCN4 epitope

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

dCas9-5xGCN4

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1B

Plasmid

#202722

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B (PIP5K1B Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-NbV5

Plasmid

#201490

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

HiBiT-NbV5

Tags

HiBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g7

Plasmid

#80790

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9-polyA

Plasmid

#72602

Purpose

express hCas9 byT7 promoter with polyadenine tails

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

Insert

Cas9-polyadenine

Tags

81-bp polyadenylation

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pQC-mCherryFP-GFP

Plasmid

#129102

Purpose

mCherryFP positive control

Depositor

Fangliang Zhang

Article

Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

Insert

mCherryFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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