We narrowed to 16,842 results for: puromycin

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pEBTetD-SNAP-CPAP

Plasmid

#136816

Purpose

Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CENPJ (CENPJ Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Cep76

Plasmid

#136814

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep76 (CEP76 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-α-tubulin

Plasmid

#136792

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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miR290-SE-RGM-tdTomato targeting vector

Plasmid

#133047

Purpose

Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80D

Plasmid

#129516

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Aspartic acid

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80F

Plasmid

#129517

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Phenylalanine

Promoter

cmv

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO1 ts2

Plasmid

#115851

Purpose

AGO1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO1 shRNA (AGO1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

Plasmid

#105022

Purpose

Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Apc (Apc Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

Plasmid

#105028

Purpose

Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Slc25a51 (Slc25a51 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

Plasmid

#105029

Purpose

Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Slc25a51 (Slc25a51 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

Plasmid

#105035

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

Plasmid

#105017

Purpose

Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Kcmf1 (Kcmf1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [dRING]

Plasmid

#126549

Purpose

Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RING domain deletion mutant [dRING mutation is a …

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

Plasmid

#123324

Purpose

Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

Plasmid

#123325

Purpose

Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1-W300A

Plasmid

#123301

Purpose

Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

W300A

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1

Plasmid

#123300

Purpose

Mammalian expression of PNRC1 C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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