We narrowed to 81,391 results for: ELL

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pKM60

Plasmid

#154159

Purpose

Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Mutation

4 wt NF-κB sites were replaced with Rel1A-specifi…

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
Clim-DC2-NMM-tdtomato

Plasmid

#129557

Purpose

pClimDC-NMM:tdT cell membrane marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

N-Myristoylation motif (NMM) fused to tdtomato

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf117

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pMP71-mtGFP-llo-lama4-alg8

Plasmid

#174605

Purpose

expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4

Expression

Mammalian

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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BRD4 CRISPRi plasmid

Plasmid

#154890

Purpose

Human BRD4 CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

BRD4-targeting gRNA

Use

CRISPR and Lentiviral

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18b

Plasmid

#135554

Purpose

Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18b (Stxbp2 Rat)

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-OsMIR390-B/c

Plasmid

#61468

Purpose

Gateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Use

Gateway entry vector

Mutation

O. sativa MIR390 precursor sequence including a c…

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LplA(AAG)

Plasmid

#61820

Purpose

Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

GFP

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAcrF1

Plasmid

#89233

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF1

Tags

6xHis-TEV and FLAG

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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mNeongreen-pGBD(PKN1 codon optimized)

Plasmid

#129634

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding doamin of PKN1(30 - 100) codon optimized

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17WHC

Plasmid

#118839

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17WHC (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

WHC: L46W, V53H, E57C

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD6A (OTU, aa 128-288)

Plasmid

#61417

Purpose

Expresses human OTUD6A (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-127.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only