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AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

Plasmid

#190112

Purpose

AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Inserts

MMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)

Use

AAV

Tags

bpNLS and bpNLS-P2A-eGFP

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

EFS and U6, H1

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1013-luc2

Plasmid

#227107

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1013

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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AAV hSYN DIO mGluR1-myc-FLAG

Plasmid

#166227

Purpose

AAV expressing cre-dependent mGluR1-myc-FLAG

Depositor

Jason Aoto

Article

Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

Insert

metabotropic glutamate receptor 1 (Grm1 Mouse)

Use

AAV and Cre/Lox

Tags

Myc and FLAG

Expression

Mammalian

Promoter

human Synapsin

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-CD8aStalk-PDGFR

Plasmid

#240242

Purpose

Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

Plasmid

#170163

Purpose

Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Has Service

AAV1

Insert

ChroME2s-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-IgG4hinge-PDGFR

Plasmid

#240245

Purpose

Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184V

Plasmid

#106175

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

hSynapsin

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

Plasmid

#233039

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3G-XRI-FLAG

Plasmid

#250671

Purpose

Timestamp monomer for encoding time along the CytoTape-vivo fiber for mouse brain in vivo applications.

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

XRI-FLAG

Use

AAV

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EGFP.T2A.NCLX

Plasmid

#181873

Purpose

Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Inserts

solute carrier family 8 member B1 (Slc8a1 Mouse)
EGFP

Use

AAV and Adenoviral

Tags

HA and myc

Expression

Mammalian

Promoter

synthetic hybrid CAG promoter and synthetic hybri…

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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MtPT4-p3

Plasmid

#160003

Purpose

Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Inserts

DsRed (reverse orientation)
DODAα1
CYP76AD1
cDOPA5GT

Expression

Plant

Promoter

Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 4-1BB]

Plasmid

#218336

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Expression

Mammalian

Promoter

TREtight

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTR6-SNAPf-TRIP

Plasmid

#189617

Purpose

Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6-TRIP_alpha

Use

AAV

Tags

alphaTag in the N-term of HTR6, SNAP-tag in the C…

Promoter

TRE

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-flex-iGABASnFR2-WPRE

Plasmid

#218880

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Vamp3-pHluorin

Plasmid

#190152

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp3 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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