165,839 results

1. Multiplex CRISPR/Cas9 Assembly System Kit

   Plasmid Kit

   #1000000055

   Purpose

   Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

   Depositor

   Takashi Yamamoto

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-mito-ZapCY1

   Plasmid

   #58996

   Purpose

   Mitochondrial Zn-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Park et al ACS Chem Biol. 2012 Aug 10.

   Insert

   mito-ZapCY1 high affinity Zn(II) sensor
   

   Use

   Tags

   See comment

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

   Plasmid

   #135391

   Purpose

   Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

   Depositor

   Fan Wang

   Insert

   TeLC-P2A-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. plRL2

   Plasmid

   #163631

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-GFP (AAV8)

   Viral Prep

   #37825-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pT3TS-RfxCas13d-NLS-HA

   Plasmid

   #141321

   Purpose

   Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d (NLS-RfxCas13d-NLS)
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

   Plasmid

   #234046

   Purpose

   expresses LaccID on the mammalian cell surface

   Depositor

   Alice Ting

   Article

   Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

   Insert

   LaccID-CD4TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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