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LCV2_mCherry

Plasmid

#155096

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3

Plasmid

#155095

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 1

Plasmid

#42543

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-OGDH_wobble

Plasmid

#110938

Purpose

OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

OGDH wobble cDNA (OGDH Human)

Use

Lentiviral

Mutation

15 silent wobble mutations corresponding to three…

Promoter

SV40

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-p35S(rc)2_4_5-GFP

Plasmid

#127520

Purpose

Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanCOL2A1enhancer

Plasmid

#173961

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region from COL2A1 gene

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001149021)

Plasmid

#77864

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

Insert

desmoglein-3 (Dsg3 Mouse)

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV5

Plasmid

#74984

Purpose

Constitutive ETV5 ORF (codon optimized), blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Inserts

ETV5 (ETV5 Human)
ETV5 (ETV5 Human)

Expression

Mammalian

Mutation

Codon optimized (synonymous)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCerulean-Rab11

Plasmid

#140574

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Inserts

CIB1 (CIB1 Mustard Weed)
Rab11A (RAB11A Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Stx5S-mCherry

Plasmid

#165087

Purpose

Expresses Stx5S-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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TRAV26-2

Plasmid

#123399

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV26-2 (TRAV26-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

Plasmid

#106180

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV5

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS

Plasmid

#127656

Purpose

Over-express NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Flag

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV7-9

Plasmid

#123437

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV7-9 (TRBV7-9 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV26-1

Plasmid

#123398

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV26-1 (TRAV26-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7-W

Plasmid

#159292

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 (KAT7 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10)

Promoter

human EF1a promoter

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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TCF7L2_pLX307

Plasmid

#98373

Purpose

Lentiviral expression of TCF7L2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Isoform X25

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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