We narrowed to 4,746 results for: MLS

1. pET15b-GreBdm-D41N

   Plasmid

   #129691

   Purpose

   express E. coli GreB E82C/C68S/D41N

   Depositor

   Jeff Gelles

   Article

   Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GreB E82C C68S D41N (greB E. coli)
   

   Use

   Tags

   his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b-GBdm

   Plasmid

   #129690

   Purpose

   express E. coli GreB E82C/C68S

   Depositor

   Jeff Gelles

   Article

   Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GreB E82C C68S (greB E. coli)
   

   Use

   Tags

   his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-20b-T7-Ntx

   Plasmid

   #58714

   Purpose

   Expresses T7-Notexin in Escherichia coli

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HOXB6-FLAGA-pSP65

   Plasmid

   #20994

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   In vitro trans/trans

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Integrin β5-GFP

   Plasmid

   #205090

   Purpose

   Express GFP-tagged human integrin β5 in mammalian cells

   Depositor

   Bianxiao Cui

   Article

   Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

   Insert

   Integrin beta 5 (ITGB5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-CAG-SPOTlight-U2GCR

   Plasmid

   #164819

   Purpose

   Reporter to measure ISR state-dependent protein synthesis

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   SPOTlight-U2GCR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Integrin β8-GFP

    Plasmid

    #205093

    Purpose

    Express GFP-tagged human integrin β8 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 8 (ITGB8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCerulean3-TOMM20-N-10

    Plasmid

    #55449

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

17. mCerulean3-Mito-7

    Plasmid

    #55433

    Purpose

    Localization: Mitochondria, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

18. mCerulean3-ER-5

    Plasmid

    #55418

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-CKAR2

    Plasmid

    #123348

    Purpose

    Improved FRET-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    CKAR2
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

    Plasmid

    #207667

    Purpose

    Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight-RA [T152E]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge