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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32,084 results for: multi

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Showing: 10181 - 10200 of 32084 results

  2. pMA-SpCas9-g7

    Plasmid
    #80790
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80790-plasmid-map-sequence-id-161640

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  3. pMA-SpCas9-g6

    Plasmid
    #80789
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80789-plasmid-map-sequence-id-161638

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  5. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  8. Salsa-BC1-P1A1

    Plasmid
    #202842
    Purpose
    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1
    Depositor
    Noah Palm
    Publication
    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP/BC1
    Expression
    Mammalian
    Promoter
    Tet Response Element (TRE)
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202842-plasmid-map-sequence-id-413389

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  9. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Publication
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  12. EKARet-nuc

    Plasmid
    #96896
    Purpose
    FLIM sensor for ERK activation in nucleus
    Depositor
    Ryohei Yasuda
    Publication
    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLIM Sensor for ERK Activity in Nucleus
    Tags
    EGFP and sREAChet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    96896-plasmid-map-sequence-id-182390

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  13. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

    Plasmid
    #79902
    Purpose
    Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus
    Depositor
    Yannick Doyon
    Publication
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG_Twin_Strep_EZH2
    Use
    CRISPR and TALEN
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Promoter
    PGK1
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    79902-plasmid-map-sequence-id-149779

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  14. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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  19. M-tdTom-ST1

    Plasmid
    #48678
    Purpose
    Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9
    Use
    CRISPR
    Promoter
    Sp6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
Showing: 10181 - 10200 of 32084 results