We narrowed to 11,432 results for: 110

1. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 902 pLNCX myr HA Akt1 K179M

   Plasmid

   #9006

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   K179M. kinase dead.

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nxph4-2A-CreERT2 Targeting Vector

   Plasmid

   #62219

   Purpose

   Targets CreERT2 to the mouse Nxph4 stop codon

   Depositor

   Hongkui Zeng

   Article

   Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

   Insert

   Nxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Nxph4

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

   Plasmid

   #224500

   Purpose

   Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB-GFP-Gab1(Y628F/Y660F)

   Plasmid

   #188658

   Purpose

   FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 1 (Gab1 Mouse)
   

   Use

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y628F and Y660F

   Promoter

   CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SYKA-c020

   Plasmid

   #175489

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-EXO1a

   Plasmid

   #111629

   Purpose

   Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

   Insert

   Exonuclease 1a (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2K-LRL-IRES-Luc2

   Plasmid

   #109230

   Purpose

   For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   LRL and Luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-DDX54-FLAG/HA

   Plasmid

   #97062

   Purpose

   Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sgNeo/Cre

    Plasmid

    #67594

    Purpose

    Expresses an Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgNeo
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLex 983-Micu2

    Plasmid

    #50372

    Purpose

    Expression in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

    Insert

    MICU2 (MICU2 Human)
    

    Use

    Lentivirus

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. PB-Neo-TetO-Cdk12_Untagged

    Plasmid

    #127178

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TetO Promoter

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-GFP-Gab2(Y604F/Y634F)

    Plasmid

    #188659

    Purpose

    FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 2 (Gab2 Mouse)
    

    Use

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y604F and Y634F

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MED19

    Plasmid

    #15420

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED19 (Med19 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-CTSS

    Plasmid

    #172415

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Cathepsin S (CTSS Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME Actin Vhh Halo Tag (JDW 1223)

    Plasmid

    #224498

    Purpose

    Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-Halo-Tag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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