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pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG1

Plasmid

#193799

Purpose

Protein production of ChemoG1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG2

Plasmid

#193800

Purpose

Protein production of ChemoG2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG3

Plasmid

#193801

Purpose

Protein production of ChemoG3 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG3

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG4

Plasmid

#193804

Purpose

Protein production of ChemoG4 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG4

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S468A

Plasmid

#27037

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

S468A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Article

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
Lv EF1a MS2-Fkbp1x 2A Hygro

Plasmid

#102806

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Article

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pCS2+-rasmKate

Plasmid

#105938

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

mKate

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET16

Plasmid

#107442

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET16 (MET16 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔ+/-

Plasmid

#35378

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 575-611

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Septin 11 isoform III

Plasmid

#120319

Purpose

Septin 11 isoform III mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ339

Plasmid

#117501

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-FKBP-HA no-pA

Plasmid

#82598

Purpose

HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FKBP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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UBC1cer60G

Plasmid

#161016

Purpose

ubiquitously weakly expressed 60-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

60meric GFP nanocage

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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CTAG_mScarlet-I

Plasmid

#136118

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG-mScarletI(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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