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PHKA1 gRNA (BRDN0001144977)

Plasmid

#77838

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA1 (PHKA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4938

Plasmid

#107527

Purpose

Expresses Fn crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_2

Plasmid

#160778

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_1

Plasmid

#160777

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEBG-IFNAR2(265-515)

Plasmid

#12437

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

IFNAR2 a.a. 265-515 (IFNAR2 Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-A3A-seBE4max-IRES

Plasmid

#174698

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

A3A-seBE4max-IRES (APOBEC3A Human)

Tags

myc tag

Expression

Mammalian

Mutation

A3A-M13I, UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147780)

Plasmid

#80226

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAT15546_nABE8e-SuperFi-Cas9

Plasmid

#184376

Purpose

Mammalian expression of SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 PARP2 E614Q

Plasmid

#192545

Purpose

pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)

Use

Gateway entry vector

Mutation

changed Glu 614 to Gln

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTE4560

Plasmid

#107526

Purpose

Expresses As crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE2

Plasmid

#160766

Purpose

Suppress POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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WEE1 gRNA (BRDN0001149349)

Plasmid

#77367

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WEE1 (WEE1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p260 pCMV-CRE-M-AR(wt)

Plasmid

#12494

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

CRE-M and AR ligand binding domain

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001146465)

Plasmid

#77174

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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