We narrowed to 12,237 results for: nsf

Showing: 10321 - 10340 of 12237 results

Results per page:

Sort results by:

pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS5-dTom

Plasmid

#178719

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS6-dTom

Plasmid

#178720

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS4-GFP

Plasmid

#178712

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

Plasmid

#155195

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 276-315

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Mutation

G144A, Y145A, G146A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn iCyt_BrEt_SnFR_PM

Plasmid

#177757

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-BUBR1-dKARD

Plasmid

#114053

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-dKARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-IBV-3CL-C143A

Plasmid

#160289

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C143A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Pf92-bio

Plasmid

#47728

Purpose

Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised Pf92

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1990

Plasmid

#49121

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC8_PH-6His-Nter-GWs-Lox (VE5587)

Plasmid

#163768

Purpose

Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

N-terminal 6His tag

Tags

6 His Tag

Expression

Insect

Promoter

PH

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC8_MF-PH-10His-Cter (VE5631)

Plasmid

#161802

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

C-terminal 10 His tag

Tags

10 His

Expression

Insect

Promoter

p10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

Plasmid

#139770

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

C-terminal TwinStrep tag

Tags

3C - TwinStrep tag

Promoter

PH

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge