We narrowed to 10,946 results for: ESP

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hRREB1-RFP

Plasmid

#22924

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pEND-349_Cacnes_ori

Plasmid

#225604

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

Plasmid

#115516

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Promoter

UAS

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_sfGFP3TCG4TAG

Plasmid

#174518

Purpose

Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

sfGFP

Tags

6 x His

Expression

Bacterial

Mutation

Recoded. TCG and TAG codons were introduced in po…

Promoter

araBAD

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

Plasmid

#117720

Purpose

Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

mAID-nanobody
IBB-mCherry

Tags

importin-beta bining domain (IBB) and triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV and None, IRES-driven

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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sq5

Plasmid

#24499

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gs alpha (Gnas Rat)

Tags

HA (see comments)

Expression

Mammalian

Mutation

This is Gs alpha with the C-terminal amino acids …

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4CNW)RS

Plasmid

#222871

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSKA413

Plasmid

#80381

Purpose

Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

ccaS (complement)
ccaR (complement)
gfpmut3

Use

Synthetic Biology; Optogenetics

Tags

FLAG tag

Expression

Bacterial

Promoter

ccaR and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti HRE mutant-Luc pGK Hygro

Plasmid

#118707

Purpose

reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HREmutant-Luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pET23 P53DBD

Plasmid

#174503

Purpose

express P53 DNA binding domain (94-312) in E.coli without tag

Depositor

Stewart Loh

Article

Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

Insert

P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 basic Mut E2

Plasmid

#48748

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)

Use

Luciferase

Mutation

Mutated E-box2 sequence from CACGTT to GCTAGT

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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