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pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

Plasmid

#181792

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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C1-82 pComb3xss anti-cFos nanobody

Plasmid

#145829

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgMyc_1

Plasmid

#138324

Purpose

Express guide RNA 2 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A4

Plasmid

#132260

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A4 (SLC4A4 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV hSyn GFP-Fxr1

Plasmid

#112732

Purpose

AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

Depositor

Martin Beaulieu

Article

Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

Insert

Fxr1p (Fxr1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

hSYN

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T2 (+minintr)

Plasmid

#119205

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

Plasmid

#71218

Purpose

dCas9 fused to the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A(E752A)

Use

Lentiviral

Tags

SPdCas9-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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SLC30A10-GFP

Plasmid

#104381

Purpose

Expresses SLC30A10-GFP wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

SLC30A10 (SLC30A10 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Pak3

Plasmid

#99510

Purpose

to express Pak3 in mammalian system

Depositor

Dong-Yan Jin

Article

Chan et al Retrovirology. 2013 Apr 26;10:47. doi: 1

Insert

Pak3 (Pak3 Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM5-mSHMT1

Plasmid

#83904

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (Shmt1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnd1-Fc-His

Plasmid

#72132

Purpose

Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnd1 (Plxnd1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_HuB

Plasmid

#65758

Purpose

constitutively expresses FLAG-HA-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_ST18

Plasmid

#64844

Purpose

A lentiviral vector for the expression of mouse ST18 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

ST18 (St18 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC41

Plasmid

#62332

Purpose

sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
PCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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