Addgene

We narrowed to 18,069 results for: Met;

Showing: 2041 - 2080 of 18069 results

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pET3_HMV-His6

Plasmid

#164843

Purpose

Expression of FL human metavinculin

Depositor

Tina Izard

Article

Rangarajan et al PLoS One. 2010 May 19;5(5):e10679. doi:

Insert

VCL (VCL Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Puro

Plasmid

#100570

Purpose

hgRNA-A21 in a lentiviral backbone with puromycin resistance

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDS282

Plasmid

#34983

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ–mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAND_Int2

Plasmid

#60590

Purpose

A transcriptional AND circuit constructed by layering NOR and NOT gates

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Int2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPhlF

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAND_yfp

Plasmid

#60592

Purpose

used with pSpec to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design2

Plasmid

#100572

Purpose

FISSEQ-hgRNA-Design2 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design2

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-QF

Plasmid

#26269

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

QF (qf Neurospora crassa)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mRFP1

Plasmid

#104025

Purpose

Plasmid encoding 2ndVal mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mRFP1

Use

Low copy

Tags

Expression

Bacterial

Mutation

mRFP1 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCC48

Plasmid

#128285

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, BBa_J23100 - phlF, parS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-15-LexABD-G4MD-QFAD

Plasmid

#46105

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCis_7+8

Plasmid

#60587

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Phamret/pcDNA3

Plasmid

#51953

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pAP998-5

Plasmid

#99501

Purpose

3XFlag::tagBFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::linker::TEV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP683-2

Plasmid

#99488

Purpose

TEV::eGFP::ER::HA::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::HA::linker::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-2ndVal mNept2.5

Plasmid

#104032

Purpose

Plasmid encoding 2ndVal mNeptune2.5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mNeptune2.5

Use

Low copy

Tags

Expression

Bacterial

Mutation

mNeptune2.5 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-5-G4BDMD-QFADM1-hsp70

Plasmid

#46113

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFADM1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP576-2

Plasmid

#99481

Purpose

3XFlag::tagBFP::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS297

Plasmid

#34986

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-4-QFBDMD-G4AD-hsp70

Plasmid

#46112

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDMD-G4AD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-2-G4BDMD-QFAD-hsp70

Plasmid

#46110

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-6-G4BDMD-QFADM2-hsp70

Plasmid

#46114

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFADM2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ExRai-PKNAR

Plasmid

#226631

Purpose

Excitation-ratiometric biosensor for monitoring Protein Kinase Novel activity in living cells

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

ExRai-PKNAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Prham-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#149339

Purpose

expresses M.Ocy1ORF8430P DNA methyltransferase (M.SpeI ortholog) in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.Ocy1ORF8430P

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCC2

Plasmid

#128283

Purpose

asymmetric plasmid partitioning E. coli - negative control

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, _parS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2041 - 2080 of 18069 results

Results per page:

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