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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 1021 - 1040 of 3270 results

  1. CBE6b-SpRY

    Plasmid
    #257826
    Purpose
    CBE6b Sp Cas9 SpRY IVT plasmid
    Depositor
    Gregory Newby
    Inserts
    CBE6b SpCas9 SpRY
    CBE6b Sp Cas9 SpRY
    Expression
    Mammalian
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    257826-plasmid-map-sequence-id-513602

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  2. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid
    #101232
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G528A, V583A, E584A, D585A, N588A and Q926A
    Promoter
    T7
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101232-plasmid-map-sequence-id-197212

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  4. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  9. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  10. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid
    #68333
    Purpose
    Parvibaculum lavamentivorans Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PlCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68333-plasmid-map-sequence-id-119549

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  11. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  20. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Mutation
    D10A nickase
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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Showing: 1021 - 1040 of 3270 results