We narrowed to 6,574 results for: val

1. pX459-sgRNA2-CTCF-prom

   Plasmid

   #195104

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sgRNA1-CTCF-prom

   Plasmid

   #195103

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(DAP10)

   Plasmid

   #177904

   Purpose

   Bacterial expression of his10-tagged human DAP10 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   DAP10 (aa 70-92 only) (HCST Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-sgRNA3-RPB1-N-term

   Plasmid

   #195112

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX459-sgRNA1-RPB1-N-term

   Plasmid

   #195110

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS::AtHAP2-V5

   Plasmid

   #132958

   Purpose

   Express AtHAP2 full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   AtHAP2 (GCS1, HAP2 Mustard Weed)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1-alpha

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-PS-Sos1 (PRM) - Cys (XSB364)

   Plasmid

   #78506

   Purpose

   Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Sos1 -Cys (PRM) (SOS1 Human)
   

   Use

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZJQIBEBT003

   Plasmid

   #140053

   Purpose

   The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

   Depositor

   Jia Zhang

   Article

   Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

   Insert

   eMutS-L157C-G233C-CBM-EGFP
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   L157C, G233C

   Promoter

   T7

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SIN40C.SFFV.mTagBFP2.let-7c

    Plasmid

    #169310

    Purpose

    Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIN40C.SFFV.dTomato.miR-125b-2

    Plasmid

    #169302

    Purpose

    Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-125b-2 (MIR125B2 Human)
    

    Use

    Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX-FHH-MAPKAP1RBD/Triple Mutant

    Plasmid

    #120711

    Purpose

    Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 RBD
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 279-353, K307A, K310A, R312L

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProEX-Af2Sir2

    Plasmid

    #8380

    Depositor

    Cynthia Wolberger

    Article

    Avalos et al Mol Cell 2002 Sep;10(3):523-35.

    Insert

    Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pIS1-wt RhoA 3'UTR

    Plasmid

    #26089

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    wt RhoA 3'UTR (RHOA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

15. PET11a-Af2sir2

    Plasmid

    #8383

    Depositor

    Cynthia Wolberger

    Article

    Avalos et al Mol Cell 2002 Sep;10(3):523-35.

    Insert

    Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PET11a-Af2Sir2D103N

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SIN40C.SFFV.GFP.miR-99a

    Plasmid

    #169304

    Purpose

    Constitutive overexpression of miR-99a with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-99a (MIR99A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. K339CLM G234A/215/347

    Plasmid

    #24449

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E…

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS424-TUB2 - human TUBB6 Cterminal tail

    Plasmid

    #60403

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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