We narrowed to 23,671 results for: CRISPR

1. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK4387 - human expression plasmid for SpCas9 Cluster 5

   Plasmid

   #101191

   Purpose

   Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster5(K918A/V922A/R925A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K918A/V922A/R925A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MMW3645 - human expression plasmid for SpCas9 Cluster 3

   Plasmid

   #101187

   Purpose

   Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   T657A/G658A/W659A/R661A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMW3770 - human expression plasmid for SpCas9 Cluster 4

   Plasmid

   #101189

   Purpose

   Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   F491A/M495A/T496A/N497A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP54-5

   Plasmid

   #65599

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs12
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AP56-6

   Plasmid

   #65593

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. AP56-5

   Plasmid

   #65591

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. AP56-8

   Plasmid

   #65596

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. AP56-7

   Plasmid

   #65594

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa13
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. AP54-3

    Plasmid

    #65597

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs12
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

11. SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

    Plasmid

    #244217

    Purpose

    Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor_ShCAST_kanR

    Plasmid

    #127924

    Purpose

    Kanamycin resistance donor for ShCAST

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    KanR, R6K origin of replication
    

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWH1266-Apra-sgRNA

    Plasmid

    #218372

    Purpose

    sgRNA expression plasmid for CRISPR-intereference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    sgRNA expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3s-Cas9-HN

    Plasmid

    #104171

    Purpose

    Cas9-WT mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    Cas9-WT
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pxpr_050-wt-ltr

    Plasmid

    #182646

    Purpose

    lentiviral expression of gRNA scaffold, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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