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Showing: 10421 - 10440 of 18430 results

  3. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  5. pJAT56

    Plasmid
    #204300
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

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  7. p667-UBC-TSSK4-V5-miniTurbo_IDG-K

    Plasmid
    #189901
    Purpose
    Gateway destination clone of TSSK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TSSK4 (TSSK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189901-plasmid-map-sequence-id-374488

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  8. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid
    #189885
    Purpose
    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    COQ8A (COQ8A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189885-plasmid-map-sequence-id-374487

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  12. p663-UBC-miniTurbo-V5-ICK_IDG-K

    Plasmid
    #158187
    Purpose
    Gateway destination clone of ICK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-ICK (CILK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158187-plasmid-map-sequence-id-314663

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  14. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  16. Mouse 5' Srcap AID GFP Puro

    Plasmid
    #127903
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene
    Depositor
    Mark Groudine
    Insert
    Srcap (Srcap Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127903-plasmid-map-sequence-id-251069

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  19. pAAV-UbC-mNeonGreen

    Plasmid
    #124102
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    UbC
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124102-plasmid-map-sequence-id-240595

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Showing: 10421 - 10440 of 18430 results