We narrowed to 94,557 results for: %s

1. pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52547

   Purpose

   Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   PhiC31o integrase
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR4_FUS_C2G_R521G

   Plasmid

   #26369

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Entry vector

   Mutation

   R521G

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGEX6-Smad3 EPSM A204S

   Plasmid

   #27108

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T179V S204A S208A S213A; A204S

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

4. LEPG_shSik3.3974

   Plasmid

   #125855

   Purpose

   Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   shRNA targeting Sik3
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   MSCV-LTR

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LEPG_shSik3.308

   Plasmid

   #125878

   Purpose

   Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   shRNA targeting Sik3
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   MSCV-LTR

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP2042-1

   Plasmid

   #105244

   Purpose

   mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAE-MIBP1

   Plasmid

   #51873

   Purpose

   Expresses full length rat MIBP1 (cloned in pCAGGS)

   Depositor

   Kenshi Hayashi, Tomoko Tahira

   Article

   Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

   Insert

   MIBP1 (Hivep2 Rat)
   

   Tags

   none

   Expression

   Mammalian

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pPK1E-1+t-gfp

   Plasmid

   #48131

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMflPT-o4

   Plasmid

   #101315

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   pac resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMflST-o4

    Plasmid

    #101316

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107322

    Purpose

    Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to SaCas9

    Promoter

    CMV IE93

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

    Plasmid

    #52540

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGL3-Ogt (-178 to +233 bp)

    Plasmid

    #79266

    Purpose

    To identify the Cis and Trans acting elements that control Ogt gene transcription.

    Depositor

    Steven Jones

    Article

    Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

    Insert

    Ogt promoter element (Ogt Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 PARL-FLAG-CT S70A

    Plasmid

    #13620

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 70 to Ala

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pSN30

    Plasmid

    #100115

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6-Smad3 EPSM V179T

    Plasmid

    #27015

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T179V S204A S208A S213A; V179T

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pGEX6-Smad3 EPSM A208S

    Plasmid

    #27109

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T179V S204A S208A S213A; A208S

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6-Smad3 EPSM A213S

    Plasmid

    #27110

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T179V S204A S208A S213A; A213S

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid

    #69232

    Purpose

    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-gRNA_zebrafish_mmp21

    Plasmid

    #72890

    Purpose

    plasmid to generate guideRNA against mmp21 gene in zebrafish

    Depositor

    Nicholas Katsanis

    Article

    Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

    Insert

    zebrafish mmp21 (mmp21 Zebrafish)
    

    Use

    CRISPR

    Promoter

    T7 RNA pol

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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