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pTRE3G-GSX2-IRES2-EBF1

Plasmid

#96966

Purpose

Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)

Tags

FLAG (on EBF1)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1

Plasmid

#60540

Purpose

Donor vector for human MLK1

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway Cloning

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pPGxRF3

Plasmid

#138384

Purpose

T2A-GAL4 knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt10B

Plasmid

#35921

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-Ms-c-Myc-W136E

Plasmid

#26024

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

W136E

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

Plasmid

#224006

Purpose

AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has Service

AAV8

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14467 - pAAV-AiE2182m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4467)

Plasmid

#224043

Purpose

AiE2182m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT neurons (strong)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has Service

AAV8

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670nano3

Plasmid

#184666

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human alpha-tubulin

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD

Plasmid

#171238

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-S100A10

Plasmid

#107200

Purpose

Expresses YFP-human S100A10 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Insert

S100A10 (S100A10 Human)

Tags

Yellow Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-ThPDE5VV

Plasmid

#134778

Purpose

cGMP FRET biosensor

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

ThPDE5VV

Tags

cpVenus-Venus and mTurquoise2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-SSFV-dCas9-GFP

Plasmid

#64105

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

SSFV

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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lenti.DTR.GFP

Plasmid

#201962

Purpose

Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

Depositor

Bernhard Schmierer

Article

Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

Insert

Diptheria Toxin Receptor

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-WT_VA

Plasmid

#141327

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-WT (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Gateway Cloning

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only