We narrowed to 59,233 results for: %s

1. p-EGFP-C1-Flag-mFmr1(A)

   Plasmid

   #87913

   Purpose

   Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

   Depositor

   Stephanie Ceman

   Article

   Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   S499A

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-CAG::Nucbow

   Plasmid

   #158992

   Purpose

   Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Nucbow
   

   Use

   Tags

   All FPs are fused to H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-Tagger

   Plasmid

   #129396

   Purpose

   Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

   Insert

   rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TIA1 RRM-mCherry-iLIDx6

   Plasmid

   #103782

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-mCherry-iLIDx6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-sL7-Cre-HA-WPRE

   Plasmid

   #204488

   Purpose

   For production of driver AAV virus for Purkinje cell-specific expression

   Depositor

   Samuel Wang

   Article

   Chen et al Nature. 2022 May;605(7911):722-727. doi:

   Insert

   Cre
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   sL7

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-EGFP-C1-Flag-mFmr1(D)

   Plasmid

   #87914

   Purpose

   Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

   Depositor

   Stephanie Ceman

   Article

   Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   S499D

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS1ClacZ

   Plasmid

   #55171

   Purpose

   lacZ-reporter vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

9. amilGFP chromoprotein

   Plasmid

   #117842

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. POLArISact

    Plasmid

    #164971

    Purpose

    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Use

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hLIN28A

    Plasmid

    #47902

    Purpose

    Expresses human LIN28A in mammalian cells using retrovirus system.

    Depositor

    Shinya Yamanaka

    Article

    Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    human LIN28A (LIN28A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VEGF binding scFv G6

    Plasmid

    #111716

    Purpose

    Yeast Surface Display of G6 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6
    

    Use

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-N90-beta-catenin

    Plasmid

    #31787

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Entry vector

    Tags

    Myc

    Expression

    Mutation

    Deletion of aa 1-90- Constitutively Active

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174884

    Purpose

    Targeting vector for generating Sox2 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Sox2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SSpB-HaloTag-p63DH

    Plasmid

    #176116

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge