We narrowed to 12,148 results for: NSI;
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Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW450
Plasmid#198831PurposeVoltage-sensitive fluorescent protein.DepositorInsert15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
ExpressionWormAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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c3GIC9n
Plasmid#62192Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9 (D10A)
UseCRISPRTagsHAMutationD10A substitution (Cas9n)PromoterTRE3GAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-cortactin
Plasmid#193287Purposemammalian transient expression of mEos3.2-cortactinDepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
TagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt11
Plasmid#184512PurposeMammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent proteinDepositorAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
ABI_cPPVp
Plasmid#119212PurposeExpresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domainDepositorInsertsplit cPPVp in fusion with ABI
TagsmycExpressionMammalianPromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PYL1_nPPVp
Plasmid#119211PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nPPVp in fusion with PYL1
TagsAU1 tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PTEN 3'UTR
Plasmid#28104DepositorInsertPTEN 3'UTR (Pten Mouse)
ExpressionMammalianAvailable SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn2
Plasmid#61868PurposeTransient overexpression of Flag-Sesn2 (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_PP7-mCherry
Plasmid#107605PurposeTransient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tagDepositorInsertPP7-mCherry
UseSynthetic BiologyTagsmCherryMutationPP7: [delta]67_74 (non-aggregating)PromoterhUBCAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C147S_pQE30
Plasmid#48631PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
TagsHisTagExpressionBacterialMutationinsertion E147B, C147SAvailable SinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pEPQD2KN0908
Plasmid#177088PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)DepositorInsertP19, CrDXS2, PaGPPS1, and CrGES
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcPylRS(Y384F Y306A)
Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
ExpressionMammalianPromoterCMVAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-PM-TurboID
Plasmid#209402PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertcTP-3XHA-TurboID
TagsGFPExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only