We narrowed to 69,965 results for: TOR

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pHR_PGK_SNIPR_synRobo/Notch

Plasmid

#188341

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Robo1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJK503

Plasmid

#155380

Purpose

dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

May 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-blac

Plasmid

#32407

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pUC-STITCH

Plasmid

#129535

Purpose

STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

STITCH

Use

CRISPR; Donor template

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOPS0253

Plasmid

#115505

Purpose

Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-TVA-mCherry

Plasmid

#149295

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Mutation

Start codon removed.

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

His10

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBEG R1-ChlorR-R4 #BGV049

Plasmid

#48943

Purpose

Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Promoter

na

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE2-EF1alpha-HA-TFAP4

Plasmid

#110158

Purpose

Lentiviral vector for expression of human TFAP4 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Notch1 del-NRR

Plasmid

#188353

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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Mef2D-shRNA

Plasmid

#32900

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2d (Mef2d Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagB

Plasmid

#112755

Purpose

express Flag-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV Gsk3 sgRNA/GFP

Plasmid

#112733

Purpose

Gsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.

Depositor

Martin Beaulieu

Article

Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

Insert

GFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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RnBarr2-mTurquoise2

Plasmid

#137792

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFU-cMVIIA-PE

Plasmid

#24147

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pREG(R1-R3) #BGV141

Plasmid

#48958

Purpose

Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only