We narrowed to 9,269 results for: ef1
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Plasmid#157951PurposeLentiviral expression of HBG site 1 sgRNADepositorInsertLenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorInsertretroCHMP3
TagsHA and T2A-H2B-BFPExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138739PurposeIntegration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x0
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE
Plasmid#118279PurposeExpresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cellsDepositorInsertsmRuby2
ChR2(H134R)
UseAAV and Cre/LoxTagsSV40 NLS and YFPPromoterEF1 alphaAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)
Plasmid#117139PurposeExpression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFPDepositorInsertHA-Dmap1 (mutagenised) (Dmap1 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationmodified BFP, modified Dmap1 CDSPromoterhuman U6 promoter, human EF1a promoterAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115891PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterEF1α promoter (1.1kb short version)Available SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75243PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD
Plasmid#114371Purposeexpresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartmentDepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2
Plasmid#161543PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2
Plasmid#161533PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75234PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75235PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC
Plasmid#114374Purposeexpresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartmentDepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75242PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75238PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75239PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1_-EGFR-FCRL3-140aa-Chimera-puro
Plasmid#248151PurposeLentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its full-length cytoplasmic tail, fused to the extracellular domain of EGFR.DepositorAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1_-EGFR- FCRL3-50aa-Chimera-puro
Plasmid#248150PurposeLentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-644; first 50 residues), fused to the extracellular domain of EGFR.DepositorAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro
Plasmid#248149PurposeLentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-687; first 93 residues), fused to the extracellular domain of EGFR.DepositorAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti
Plasmid#239610PurposeConstitutive active CRISPRgenee construct with a blasticidin resistanceDepositorInsertZIM3 KRAB domain (ZIM3 )
UseCRISPR and LentiviralAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo
Plasmid#239608PurposeBackbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistanceDepositorInsertfiller-trcr-H1-filler
UseCRISPR and LentiviralAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV EF1alpha INTS4 K26/27Q IRES Cherry
Plasmid#228112PurposeLentiviral expression of human INTS4 K26/27QDepositorInsertINTS4 (INTS4 Human)
UseLentiviralExpressionMammalianMutationMutated Lysine 26 and 27 to GlutaminePromoterEF 1alphaAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-TRIP12-391-938-EGFP-Hygro
Plasmid#238092PurposeExpression of structurally predicted WWE domain of TRIP12 with EGFP on C terminalDepositorInsertTRIP12 (TRIP12 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 319-938 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-TRIP12(749-836)-EGFP
Plasmid#196229PurposeEGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged TRIP12(749- 836)
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP
Plasmid#196235PurposeEGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged Af1521(K35E/Y145R)
UseLentiviralTagsEGFPExpressionMammalianMutationK35E and Y145RPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RNF146(100-182)-myc
Plasmid#196236PurposeRNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertRNF146(100-182) encoding a C-terminal myc tag
UseLentiviralTagsmyc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc
Plasmid#196237PurposeAf1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertAf1521(K35E/Y145R) encoding a C-terminal myc tag
UseLentiviralTagsmyc tagExpressionMammalianMutationamino acid 35 lysine is replaced with glutamic ac…PromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
(942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop
Plasmid#195624PurposepAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi constructDepositorInsertdCasphi
UseAAVTagsC-Myc (NLS), HA tag, and ZIM3ExpressionMammalianMutationP749A and P753APromoterEF1a (Mlu1/EcoR1)Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB-327-Ef1-MpCKA-K151M-D258N-TagRFP
Plasmid#238530PurposePlant expression of M. polymorpha CK2 alpha K151M, D258N, tagged with TagRFP in plantsDepositorInsertMpCKA
TagsTagRFPExpressionPlantMutationK151M, D258NAvailable SinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235301PurposeComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
Plasmid#236279PurposeLentiviral vector that can express hAqp1 with fkbp12 degron in mammalian cells. EF1a promoter. EGFP marker.DepositorInserthuman aquaporin 1 (AQP1 Human)
UseLentiviralTagsFlag and fkbp12ExpressionMammalianPromoterEF1aAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235302PurposeComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE
Plasmid#237446PurposeIntersectional expression of Myc-tagged synaptophysin in the presence of Cre and FlpDepositorInsertCon/Fon-Synaptophysin-10xMyc
UseAAVExpressionMammalianPromoterEF1aAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
Plasmid#233442PurposePiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)DepositorInsertFKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export
Plasmid#233381PurposeOptimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR2(ecto+TM)-tdMCP
TagsHAExpressionMammalianMutationWTPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA
Plasmid#233040PurposeTo express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequenceDepositorInsertmouse homeostatic iron regulator with H67D mutation (Hfe Mouse)
UseAAVTagsGFPMutationH67DAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa
Plasmid#233032PurposeTo Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genomeDepositorInsertCasRx and mCherry
UseAAVTagsmCherryAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only