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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 1041 - 1060 of 2493 results

  8. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

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  11. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196711-plasmid-map-sequence-id-392561

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  12. mCh-KIF5A*-strep

    Plasmid
    #120163
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 5A (Kif5a Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF5A: 1-559 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120163-plasmid-map-sequence-id-247481

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  14. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid
    #187953
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187953-plasmid-map-sequence-id-373487

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  17. pWT065a

    Plasmid
    #107912
    Purpose
    W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Publication
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    (TCF-LEF)7-BE3
    Expression
    Mammalian
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107912-plasmid-map-sequence-id-264842

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  19. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid
    #211786
    Purpose
    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, Spy, Snoop, aGCN4, AviTags
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    dCas9 D10A and H840A
    Promoter
    pCAGG abd pEF1a
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    211786-plasmid-map-sequence-id-417395

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Showing: 1041 - 1060 of 2493 results