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Plasmid#209240PurposeMammalian expression of ATP8A1 mutant E191QDepositorInsertATP8A1 (ATP8A1 Human)
UseTagsHAExpressionMammalianMutationE191QPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOP-flash
Plasmid#83467PurposeTEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/cyto-iAspSnFR
Plasmid#201394PurposeCMV overexpression of cyto-iAspSnFR in mammalian cell linesDepositorInsertcyto-iAspSnFR
UseTagsExpressionMammalianMutationgltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…PromoterCMVAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionTagsExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-Tat
Plasmid#138478PurposeExpresses HIV Tat for efficient sgRNA packaging.DepositorInsertTat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-FLAG-dCas9-VPR
Plasmid#114196PurposeLentivirus compatible dCas9-VPR construct driven by the human SYN promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSYNAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorInsertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…ExpressionMutationPromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’-LMNA-eGFP-KI
Plasmid#170545PurposeExpresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNADepositorInsertsLeft Homology arm for LMNA knockin
Right Homology arm for VIM knockin
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-NTERM-GFP
Plasmid#185775PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-CTERM-GFP
Plasmid#185770PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX FLAG-SLC38A9.1
Plasmid#199749PurposeLentiviral construct to stably express SLC38A91.1DepositorInsertSLC38A9.1
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-CTERM-GFP
Plasmid#185776PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Elongin B-pcDNA3.1(+)-Zeo
Plasmid#20000DepositorInserttranscription elongation factor B (ELOB Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
PGK-IKZF3-CTERM-GFP
Plasmid#185779PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-CTERM-GFP
Plasmid#185778PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-HALO-CTERM-GFP
Plasmid#185777PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-NTERM-GFP
Plasmid#185771PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only