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Plasmid#79698PurposeThis plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK4 (PAK4 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK6_HUMAN_D0
Plasmid#79703PurposeThis plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK6 (PAK6 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
UseTagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
UseTags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7Available sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-NPR4 (SA binding domain)
Plasmid#226885PurposeExpresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble formDepositorInsertThe Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationNPR4 SA-binding core (residues 373-516), with a s…PromoterT7Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSJ375
Plasmid#103064PurposeExpresses ImmE1, immunity to colicin E1DepositorUseTagsExpressionBacterialMutationPromoterE1 immunity protein promoter from ColE1Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialMutationPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP HO
Plasmid#207413PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP CD
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-hDDX5/17
Plasmid#71307PurposeInducible shRNA knockdown of both human DDX5 and DDX17 genesDepositorInsertDDX5/17 shRNA (DDX17 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTetOAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_6xH
Plasmid#186461PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFAK2 (PTK2B Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK24_HUMAN_D0
Plasmid#79742PurposeThis plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK24 (STK24 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only