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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Publication

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLentiPGK Blast DEST JNKKTRmRuby2

Plasmid

#59154

Purpose

Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLVU/RED

Plasmid

#24178

Depositor

Lars Ittner

Publication

Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

Insert

Chloramphenicol resistance gene (CmR) - ccdB gene

Use

Gateway Cloning and Lentiviral

Tags

DsRED

Expression

Mammalian

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-EGFR

Plasmid

#23935

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Publication

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Publication

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Publication

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

Plasmid

#71719

Purpose

Expressing a GFP-tagged degron in the soma of C. elegans

Depositor

Abby Dernburg

Publication

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

auxin-responsive protein IAA17 (AXR3 Mustard Weed)

Tags

EmGFP

Expression

Worm

Mutation

minimal degron sequence (71-114aa) with start cod…

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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tetO-MEF2C

Plasmid

#46031

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLEX_306

Plasmid

#41391

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

Depositor

David Root

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

PGK

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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