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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL MND WASp

Plasmid

#36248

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

WASp (WAS Human)

Use

Lentiviral

Promoter

MND

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX303-GFP

Plasmid

#162032

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE337

Plasmid

#153246

Purpose

M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-BirA-HA

Plasmid

#232587

Purpose

Express BirA tagged to the C-terminus of CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-1

Plasmid

#163127

Purpose

pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Ubiquitin

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-hGRK4

Plasmid

#32690

Depositor

Robert Lefkowitz

Article

Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

Insert

GRK4 (GRK4 Human)

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
FUW-cFos

Plasmid

#193089

Purpose

constitutive expression of mouse cFos in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

cFos

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eLACCO1.1

Plasmid

#168790

Purpose

Astrocyte expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLacco1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

GFAP

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-WPRE

Plasmid

#202613

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-85b

Plasmid

#53282

Purpose

Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, crtY, crtI

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJE53

Plasmid

#194153

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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