We narrowed to 80,174 results for: ELL

1. pCsy_complex

   Plasmid

   #89232

   Purpose

   Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Inserts

   Csy1
   Csy2
   Csy3
   Csy4
   

   Tags

   6xHis-TEV protease

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEND-304_Cacnes_tetR

   Plasmid

   #225619

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEND-375_Cacnes_phlF

   Plasmid

   #225620

   Purpose

   C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   PhlF
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-226_Cacnes_mCitrine

   Plasmid

   #225613

   Purpose

   Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCitrine
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Meis1

   Plasmid

   #131605

   Purpose

   expression of mouse Meis1 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Meis1 (Meis1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Solo-mStayGold-MYO1C

   Plasmid

   #227306

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a_CD MUT TET1

   Plasmid

   #124083

   Purpose

   Expression of mutated catalytic domain only form of TET1

   Depositor

   Silvia Monticelli

   Article

   Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

   Insert

   TET1 - catalytic domain mutated (TET1 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1672Y, D1674A

   Promoter

   EF1 alpha

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1071 pBabe puroL myr Akt T308A S473A

   Plasmid

   #9014

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 T308A S473A (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   T308A S473A

   Available Since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

    Plasmid

    #104097

    Purpose

    Expresses TREAT-PK co-localization control in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

    Plasmid

    #192067

    Purpose

    Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for human FXOP2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJZC33

    Plasmid

    #62330

    Purpose

    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+-Pard3-EGFP-PIF6

    Plasmid

    #154914

    Purpose

    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).

    Depositor

    Clare Buckley, Jonathan Clarke

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    1-100 amino acids of Arabidopsis PIF6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

    Plasmid

    #136514

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    Codon-optimized AcrIIA5
    

    Use

    Lentiviral

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. VanGlow-RR-DSCP

    Plasmid

    #83339

    Purpose

    for Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    mCherry::Renilla(nls)

    Expression

    Insect

    Promoter

    Drosophila Synthetic Core Promoter

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV neo WT Akt1

    Plasmid

    #14549

    Depositor

    Joan Brugge

    Article

    Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pRK5 FLAG DEPTOR (PDZ domain)

    Plasmid

    #21701

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR PDZ domain (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PDZ domain only

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Puro-TDP-43-A90V

    Plasmid

    #133754

    Purpose

    Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 90 to valine

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Puro-TDP-43-P112H

    Plasmid

    #133760

    Purpose

    Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed proline 112 to histidine.

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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