We narrowed to 80,174 results for: ELL

1. pHR RPS27A K107/113R-3xFlag_IRES-GFP

   Plasmid

   #198392

   Purpose

   RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   ubiquitination site double mutant (K107/113R)

   Promoter

   EF1A

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-LplA(AAG)

   Plasmid

   #61307

   Purpose

   Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Tags

   6xHis, Flag, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. VanGlow-GL-DSCP

   Plasmid

   #83338

   Purpose

   for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

   Depositor

   Cheng-Yu Lee

   Article

   Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   GFP::Luciferase(nls)

   Expression

   Insect

   Promoter

   Drosophila Synthetic Core Promoter

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-TAG-HES7

   Plasmid

   #130935

   Purpose

   PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   HES7 (HES7 Human)
   

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2/GD-IRES-GFP-CTNNB1

   Plasmid

   #192868

   Purpose

   Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

   Depositor

   David Largaespada

   Article

   Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

   Inserts

   luc+
   EGFP
   CTNNB1
   

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH4R-P2A-mCherry-N1

   Plasmid

   #84333

   Purpose

   Co-expresses (untagged) Histamine-4 receptor and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H4R-P2A-mCherry (HRH4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-hK + E2-Crimson

   Plasmid

   #74946

   Purpose

   Dosage control and tracing of reprogramming episomes

   Depositor

   Ann Zovein

   Article

   Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

   Insert

   Klf4 (KLF4 Human)
   

   Expression

   Mammalian

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pPB-CAG-3xFLAG::Fnip1-pgk-hph

   Plasmid

   #48758

   Purpose

   PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   3xFLAG::Fnip1 (Fnip1 Mouse)
   

   Use

   Piggybac

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJZC32

    Plasmid

    #62327

    Purpose

    sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH3R-P2A-mCherry-N1

    Plasmid

    #84332

    Purpose

    Co-expresses (untagged) Histamine-3 receptor and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H3R-P2A-mCherry (HRH3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pf113-bio

    Plasmid

    #47729

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf113
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

    Plasmid

    #214877

    Purpose

    Lentiviral vector encoding RfxCas13d targeting LPT guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crLPT-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP-KCC2

    Plasmid

    #104075

    Purpose

    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

    Depositor

    Igor Medyna

    Article

    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    CMV

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.2/V5-DEST NET1A V5 WT

    Plasmid

    #69817

    Purpose

    Expresses NET1A-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    NET1A (NET1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

    Plasmid

    #174894

    Purpose

    AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AnapRS
    

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AdMKP-3 S197A

    Plasmid

    #131522

    Purpose

    Expresses MKP-3 with S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    changed Serine 197 to Alanine

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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