165,817 results

1. pmiHiFiCas9

   Plasmid

   #164832

   Purpose

   miHiFiCas9 expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miHiFiCas9 (contains Brex27 at sequence 5031-5138).
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   change R 691 to A

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMU-TPr

   Plasmid

   #61189

   Purpose

   Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtTIP1-1
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-GFP-TurboID-NES_pLX304

   Plasmid

   #202019

   Purpose

   expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-GFP-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

   Plasmid

   #159914

   Purpose

   Mutagenesis of Rosa26 with SauCas9

   Depositor

   Larry Zweifel

   Insert

   Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6MFV SLC10A1

   Plasmid

   #194130

   Purpose

   Expresses NTCP (SLC10A1) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC10A1 (SLC10A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. JD150

   Plasmid

   #229485

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, E4orf6, L4-22k/33k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-L1PA1-CH-mneo

   Plasmid

   #51288

   Purpose

   contains the fully codon optimized ORF1 and ORF2 of the human L1RP

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

   Insert

   L1 (L1RE1 Human)
   

   Use

   Tags

   mneoI indicator cassette containing an inverted n…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPD95_75

    Plasmid

    #1494

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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