We narrowed to 13,693 results for: crispr cas9

1. pMJ841

   Plasmid

   #39318

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   T7

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMK288 (mAID-Bsr)

   Plasmid

   #72826

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Bsr
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ePE2

   Plasmid

   #202029

   Purpose

   Prime Editing plasmid for tag insertion

   Depositor

   Pengcheng Wei

   Article

   Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

   Insert

   Cas9，
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKL2013

   Plasmid

   #172182

   Purpose

   A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

   Depositor

   Kan Wang

   Article

   McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCLV3Cas-LT0

   Plasmid

   #210548

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
   

   Expression

   Plant

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUF-AsCpf1-pre-gRNA

   Plasmid

   #137849

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1 and pre-gRNA cassette
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT25_GalL_BE3

   Plasmid

   #143736

   Purpose

   Expresses BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUF-LbCpf1-pre-gRNA

   Plasmid

   #137848

   Purpose

   LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   LbCpf1-NLS-FLAG and gRNA cassette
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hDNMT3B_KO_puro

    Plasmid

    #234998

    Purpose

    Stable knockout of DNMT3B function in mammalian cells

    Depositor

    Samrat Roy Choudhury

    Article

    Choudhury et al Genes & Diseases. 2025 Sep 6; 101843. do

    Insert

    hCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid

    #184820

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    rln3a-HA
    

    Use

    CRISPR; For integration of qf2 by homology-direct…

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEL683

    Plasmid

    #205593

    Purpose

    Cas9-PE2 for prime editing in rice

    Depositor

    Yong Zhang

    Article

    Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    ZmUbi

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330.puro sgRNA KGA Stop1

    Plasmid

    #110405

    Purpose

    CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCZGY2750

    Plasmid

    #135094

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr IV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    sgRNA for cxTi10082 (actgttggatgcctgtgtag)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR1CB0008

    Plasmid

    #117544

    Purpose

    Level 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSmart-Nm-sgRNA-BbsI

    Plasmid

    #49157

    Purpose

    Plasmid for cloning spacer into sgRNA for NmCas9

    Depositor

    James Thomson

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORFE0005

    Plasmid

    #112073

    Purpose

    AtDeadCAS9 D10A H840A without stop codon module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A without stop codon
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TLCV2 - sgPTEN

    Plasmid

    #202759

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting PTEN
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HiFi iFE

    Plasmid

    #190141

    Purpose

    Expresses HiFiCas9-POLB (HiFi iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge