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pSin-NE1m

Plasmid

#172883

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

J. Julius Zhu

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Insert

NE SENSOR

Expression

Mammalian

Promoter

sp6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LentiU6-LacZ-GFP-Puro (BB)

Plasmid

#170459

Purpose

For cloning gRNA block

Depositor

Yonglun Luo

Article

Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

Insert

hU6-BB-LacZ and EF1A-EGFP-2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 for gRNA and EF1A for EGFP-2A-Puro

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXQ1

Plasmid

#101628

Purpose

Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXQ1 (FOXQ1 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_1

Plasmid

#160762

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Plasmid

#159541

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-c

Plasmid

#108540

Purpose

Expresses human PCDH7 transcript variant c in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant c (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-b

Plasmid

#108539

Purpose

Expresses human PCDH7 transcript variant b in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant b (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS16

Plasmid

#107930

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS11

Plasmid

#107925

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL17

Plasmid

#107923

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSc1

Plasmid

#80436

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pmiR-96 cluster promoter

Plasmid

#62517

Purpose

microRNA 183-96-182 cluster promoter luciferase plasmid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

Insert

promoter of microRNA-183-96-182 cluster

Tags

RenSP (optimized renilla luciferase gene)

Expression

Mammalian

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pBullet-vac-c

Plasmid

#53076

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OTmut (AAV1)

Viral Prep

#185388-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Promoter

Syn

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_OTmut (AAV9)

Viral Prep

#185388-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Promoter

Syn

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only
LV2btCRISPRko.v1

Pooled Library

#213927

Purpose

Uses lentivirus to deliver the library into hard-to-transduce cells

Depositor

Wenfang Spring Tan

Article

Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only