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pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

Plasmid

#205276

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

T513K

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

Plasmid

#205274

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D487K

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

Plasmid

#205272

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D300R

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCgVchCAST-crtYf

Plasmid

#203816

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SARS-CoV-2_PLP

Plasmid

#203509

Purpose

Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-KUNV_NS2B-GS-NS3

Plasmid

#203506

Purpose

Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

KUNV NS2B-GS-NS3

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Mutation

S100L (C299T)

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2b

Plasmid

#186819

Purpose

Fluorescent reporter for Camk2b expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2b promoter (Camk2b Mouse)

Use

Luciferase

Promoter

Camk2b promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGl3-Camk2g

Plasmid

#186821

Purpose

Fluorescent reporter for Camk2g expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2g promoter (Camk2g Mouse)

Use

Luciferase

Promoter

Camk2g promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP

Plasmid

#190879

Purpose

Cre-on expression of soma-targeted QuasAr6b under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

soma-targeted QuasAr6b

Use

AAV

Tags

EGFP

Promoter

hSyn

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb6m

Plasmid

#184680

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb6m (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_miR-290-295KO-gRNA1

Plasmid

#172709

Purpose

Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_miR-290-295KO-gRNA2

Plasmid

#172710

Purpose

Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-puro-hPKAalpha

Plasmid

#185379

Purpose

For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha

Depositor

Ayaz Najafov

Insert

PRKACA (PRKACA Human)

Expression

Mammalian

Mutation

WT

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Mutation

E66T

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Sc eRF1 (WT)

Plasmid

#175413

Purpose

Encodes His-MBP-TEV-WT eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2xVlk1-3xFlag

Plasmid

#172773

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

Insert

Vlk1 (pkdcc.1.S Frog)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR5 Mm ATP13A2

Plasmid

#171822

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR3 Mm ATP13A2

Plasmid

#171771

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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