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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE497

Plasmid

#153249

Purpose

M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H CC editing reporter

Plasmid

#198885

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGC)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSPPac-hp

Plasmid

#48123

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of Pac

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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114_UBI_iCre_crystGFP

Plasmid

#171797

Purpose

Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised iCre under the control of the Ubiquitin promoter

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
tdTomato-β-actinCDS3

Plasmid

#183715

Purpose

tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence

Use

Lentiviral

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPA1-Halo

Plasmid

#175307

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSRalpha-MSV-mouse Cdk4wt-Tk-neo

Plasmid

#115525

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

Insert

Mouse Cdk4 (Cdk4 Mouse)

Use

Retroviral

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only
pPB-PNGT3-d2EGFP_CMV-mCherry

Plasmid

#213765

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

PNGT#3-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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