We narrowed to 15,044 results for: invs

1. pcDNA5/FRT/TO DNAJB6a

   Plasmid

   #19475

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB6a (DNAJB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CHRFAM7ADelta2bp

   Plasmid

   #62515

   Purpose

   mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

   Depositor

   Henry Lester, Sherry Leonard

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7ADelta2bp (CHRFAM7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partially duplication of CHRNA7 with 2-bp deletio…

   Promoter

   CMV

   Available Since

   March 20, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. HA-CK2alpha’ V67A/I175A (pJD24)

   Plasmid

   #37363

   Depositor

   David Litchfield

   Article

   Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

   Insert

   CK2α' V67A/I175A (CSNK2A2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V67A, I175A

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

4. qTAG-N-Blast-sTagRFP-TUBA1B

   Plasmid

   #207768

   Purpose

   Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA5-FRT-T0-Flag-HROB

   Plasmid

   #135298

   Purpose

   Expresses Flag-tagged HROB in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

   Insert

   HROB (HROB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Opto-GPR161

   Plasmid

   #106055

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR161; Opto-GPR161; E9)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR161 (GPR161 Bovine, Human)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

   Plasmid

   #51257

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248,789,805,817,848,9…

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PCRII apoEb

   Plasmid

   #121901

   Purpose

   Plasmid for making zebrafish apoEb in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   Apoeb (apoeb Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

   Plasmid

   #123339

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HtrA2-FLAG S400D

    Plasmid

    #15940

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S400D

    Promoter

    Available Since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pKK-FLAG-TEV

    Plasmid

    #105768

    Purpose

    Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT TO GFP MPS1 K-R

    Plasmid

    #59819

    Purpose

    Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

    Insert

    Mps1 (TTK Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    KD, catalytically inactive, D664A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-MED11

    Plasmid

    #15418

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED11 (Med11 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pDisplay-GRAB_CRF0.5

    Plasmid

    #208654

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hHSF1 S303A

    Plasmid

    #118363

    Purpose

    This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Opto-GPR37

    Plasmid

    #106019

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37; Opto-GPR37; B9)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR37 (GPR37 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. HMGN1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176943

    Purpose

    Mammalian expression vector encoding HMGN1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    HMGN1 (HMGN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge