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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,056 results for: BASE

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Showing: 10941 - 10960 of 24056 results

  3. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

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  5. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  7. pLib6.6

    Plasmid
    #176652
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Expression
    Insect
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176652-plasmid-map-sequence-id-348545

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  11. pPuroR-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165898
    Purpose
    Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165898-plasmid-map-sequence-id-331224

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  12. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  13. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

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  15. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid
    #121841
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121841-plasmid-map-sequence-id-237395

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  16. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

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  17. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Promoter
    gpdA
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

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Showing: 10941 - 10960 of 24056 results