We narrowed to 23,036 results for: his

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pET151-GFP-PP-RPN2 (797-953)

Plasmid

#74228

Purpose

[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 mCherry Gamma 3

Plasmid

#65234

Purpose

To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

Depositor

Narasimhan Gautam

Article

Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

Inserts

mcherry
Gng3 (Gng3 Mouse)

Tags

6-His tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pETDuet-msGC-NT2

Plasmid

#74763

Purpose

expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

Depositor

William Montfort

Article

Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7

Inserts

fragment of alfa subunit of sGC
fragment of beta subunit of sGC

Tags

His-tag

Expression

Bacterial

Mutation

fragment 49-471 of the alfa subunit

Promoter

T7

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET151-hUCH37 (ISF1)(M148D/F149D)

Plasmid

#71388

Purpose

Expression of hUCH37 (M148D/F149D) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) M148D/F149D (UCHL5 Human)

Tags

HIS-TEV sequence

Expression

Bacterial

Mutation

changed methionine 148 to aspartate, phenylalanin…

Promoter

T7

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BMPR1A

Plasmid

#67105

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR1A (BMPR1A Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 EHD2

Plasmid

#67107

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EHD2 (EHD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 DM

Plasmid

#61931

Purpose

Expression of hUCH37 (ISF1) M269E/N291D in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) M269E/N291D (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Changed methionine 269 to glutamate and asparagin…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 (ISF1)(M148A/F149A)

Plasmid

#61933

Purpose

Expression of hUCH37 (M148A/F149A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Methionine 148 and Phenylalanine 149 were mutated…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter hsa-mir-128-1

Plasmid

#46743

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-128-1 (MIR128-1 Human)

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETC PITPbeta N89F

Plasmid

#58279

Purpose

bacterial expression of PITP beta N89F mutant

Depositor

Shamshad Cockcroft

Article

Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

N89F (abolishes PI binding)

Promoter

T7

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pET-GST-TNRC6A-(935-984)-NES-mut

Plasmid

#42051

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984, F951A, F955A, I958A, F960A

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-DKFZP566J2046

Plasmid

#34818

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

fumarylacetoacetate hydrolase domain containing 1 (FAHD1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-PTD012

Plasmid

#34797

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PTD012 (C11orf54 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA-E222A-E272A

Plasmid

#35060

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E222A and E272A…

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-Y218A-Y267A

Plasmid

#35059

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; Y218A and Y267A…

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E381A

Plasmid

#35058

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E381A mutation

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E272A

Plasmid

#35057

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E272A mutation

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(936-1113)

Plasmid

#35075

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 936-1113

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only