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pEN-X-hPGK-PuroR-Z

Plasmid

#218904

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-PuroR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag D)

Plasmid

#16608

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag D) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragD (PvuII->SmaI). The PvuII site is at -484…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Mutation

R691A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab14

Plasmid

#109600

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab14

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab14 (rab14 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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actc1b-lamp1-mKate2

Plasmid

#109507

Purpose

Muscle specific zebrafish expression of mKate2 fused to lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR-ADAM29i1

Plasmid

#59606

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GLB1-1/1

Plasmid

#91346

Purpose

Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GLB1-1/1 (SH3GLB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT1

Plasmid

#106304

Purpose

Express SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (TBE 1m/2m - mut)

Plasmid

#16596

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (TBE 1m/2m mut) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Tcf-4 binding elements (TBEs) mutated

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pPB-MGT1-d2EGFP_CMV-mCherry

Plasmid

#213763

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#1-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd9-1D4

Plasmid

#42271

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 9 (Fzd9 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-VinculinI997A_TurboID_Venus

Plasmid

#200274

Purpose

Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinI997A_TurboID_Venus

Expression

Mammalian

Mutation

change isoleucine to alanine at 997 of vinculin

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBacPAK p110a RBD DM

Plasmid

#55723

Purpose

His3 GST tagged RBD double mutant p110 alpha T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

His3 GST

Mutation

T207D, K227A

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pRKB134_GI-DUX4-intron2(-)

Plasmid

#83968

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(-) (DUX4 Human)

Expression

Mammalian

Promoter

mouseCMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pRKB133_GI-DUX4-intron2(+)

Plasmid

#83967

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(+) (DUX4 Human)

Expression

Mammalian

Promoter

mouseCMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only