We narrowed to 31,694 results for: PLE

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CD8a

Plasmid

#86050

Purpose

A mammalian expression plasmid encoding CD8a without tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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HA-hULK1

Plasmid

#31963

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM55261

Plasmid

#153210

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Long P2A

Plasmid

#164111

Purpose

Detect the long Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot1 (CNOT1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUltra

Plasmid

#24129

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

Depositor

Malcolm Moore

Article

Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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piggyFlex

Plasmid

#218234

Purpose

A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

Depositor

Jay Shendure

Article

Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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