We narrowed to 2,364 results for: PUC19

1. pAP686-1

   Plasmid

   #99491

   Purpose

   TEV::eGFP::ER::V5::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::V5::linker::3XFlag
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAP640-2

   Plasmid

   #99487

   Purpose

   3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag
   

   Expression

   Bacterial

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGI000

   Plasmid

   #48863

   Purpose

   Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGD006

   Plasmid

   #48836

   Purpose

   Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   SV40 NLS
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGE002

   Plasmid

   #48840

   Purpose

   Provides the WUS terminator as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS terminator
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGB005

   Plasmid

   #48822

   Purpose

   Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   SV40 nuclear localization signal
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGB006

   Plasmid

   #48823

   Purpose

   Provides an ER signal sequence as GreenGate N-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   signal sequence ER
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGA003

   Plasmid

   #48814

   Purpose

   Provides the WUS promoter as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGGA002

   Plasmid

   #48813

   Purpose

   Provides the AP3 promoter as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   AP3 promoter
   

   Use

   Golden gate compatible cloning vector

   Mutation

   internal BsaI recognition site removed by substit…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSW036

    Plasmid

    #140034

    Purpose

    KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Yellow fluorescent protein
    

    Expression

    Bacterial

    Promoter

    cpt

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAS12069_U6-pegRNA-mCherry

    Plasmid

    #177180

    Purpose

    pegRNA cloning plasmid with mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG108 - K

    Plasmid

    #178039

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Insert

    Kanamycin resistance
    

    Expression

    Bacterial

    Mutation

    ErmAM has been replaced with Kanamycin for select…

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6_N7-sgRNA2

    Plasmid

    #190664

    Purpose

    Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    N7 sgRNA2
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG106 - KA

    Plasmid

    #178041

    Purpose

    Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Type

    Empty backbone

    Use

    Shuttle vector

    Expression

    Bacterial

    Available Since

    Feb. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSCW01

    Plasmid

    #72300

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDAC569

    Plasmid

    #195241

    Purpose

    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDAS12222_U6-pegRNA-BFP

    Plasmid

    #177181

    Purpose

    pegRNA cloning plasmid with BFP marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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