We narrowed to 4,242 results for: Pet2;

1. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET23a-H6-TEV-cAbl

   Plasmid

   #214234

   Purpose

   Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

   Depositor

   Neel Shah

   Article

   Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

   Insert

   ABL1 Kinase domain (ABL1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-iLOV-Rsn2

   Plasmid

   #61687

   Purpose

   bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

   Depositor

   Brian Smith

   Insert

   Ranaspumin-2
   

   Use

   Tags

   His and iLOV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29b-Ava3858-3856

   Plasmid

   #45745

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3856
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-SUMO-SpyTag003

   Plasmid

   #216282

   Purpose

   Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SUMO-SpyTag003
   

   Use

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET26-Ufd1-His

    Plasmid

    #21266

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Ufd1 (Ufd1l Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET23-CBD-(-PP)-EGFP

    Plasmid

    #12597

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    Cdc42 Biosensor (WAS Human)
    

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    F271C

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-ETV6-ETS

    Plasmid

    #111180

    Purpose

    Minimal ETS domain of human ETV6 (TEL)

    Depositor

    Gregory Poon

    Article

    Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

    Insert

    ETV6 ETS domain (ETV6 Bovine, Human, Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge