We narrowed to 17,353 results for: She

1. pFlpStop-HDR-UAS-2.1-tdTom

   Plasmid

   #89148

   Purpose

   FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   5XUAS, 3XP3-dsRed

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER.retro.puro-shNC

   Plasmid

   #113535

   Purpose

   Control retroviral vector.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shNC (scrambled control shRNA)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAF-MutH

   Plasmid

   #86609

   Purpose

   E. coli MutH expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutH
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAF-MutS

   Plasmid

   #86605

   Purpose

   E. coli MutS expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutS (D835R,R840E)
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   D835R, R840E

   Promoter

   T7

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBC_HA_L7Ae-VP64

   Plasmid

   #68421

   Purpose

   Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   L7Ae
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   Promoter

   human UbC

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-jRCaMP1a

   Plasmid

   #113936

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jRCaMP1a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBad-HisB-GRvT

    Plasmid

    #87363

    Purpose

    Expresses GRvT, a tandem heterodimer green and red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GRvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraBad

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-R-GECO1.2

    Plasmid

    #113937

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    R-GECO1.2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. XW09

    Plasmid

    #65833

    Purpose

    Punc-4c-QS-SL2-mcherry3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Neurospora crassa OR74A repressor (qa-1S)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4c

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.11 promPDE4D3

    Plasmid

    #27302

    Depositor

    David E. Fisher

    Article

    Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

    Insert

    prom PDE4D3 (PDE4D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ERAP1

    Plasmid

    #39174

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Baculovirus expression

    Tags

    TEV-His10-Flag

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only