We narrowed to 18,398 results for: Mut

1. pCS-lpp0.2-K64A

   Plasmid

   #201789

   Purpose

   mutant of PadR; transcriptional regulator can respond to p-coumaric acid

   Depositor

   Yajun Yan

   Article

   Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

   Insert

   PadR (padR Bacillus subtilis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K64A

   Promoter

   Available Since

   July 12, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMUH_unc84_2XGFP

   Plasmid

   #46023

   Depositor

   Sean Eddy

   Article

   Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

   Insert

   UNC-84 (unc-84 )
   

   Use

   10xuas-phi31-expression vector

   Tags

   2XGFP

   Expression

   Mutation

   Two amino acid mutations (F279L and L500P) compar…

   Promoter

   10XUAS

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-52: HIST1H2BJ-mEGFP

   Plasmid

   #109121

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ

   Depositor

   Allen Institute for Cell Science

   Insert

   HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDisplay-Rncp-iGluSnFR1

   Plasmid

   #107336

   Purpose

   Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   Rncp-iGluSnFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK562 (pDS-GFP)

   Plasmid

   #183129

   Purpose

   Control plasmid that expresses off-target dsRNA

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Insert

   GFPmut3 (no RBS)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-58: NUP153-mEGFP

   Plasmid

   #114407

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153

   Depositor

   Allen Institute for Cell Science

   Insert

   NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Sept. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AICSDP-26:ST6GAL1-mEGFP

   Plasmid

   #101786

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1

   Depositor

   Allen Institute for Cell Science

   Insert

   ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. FnR8-10

   Plasmid

   #182569

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. cfSGFP2-N

   Plasmid

   #37533

   Depositor

   Ikuo Wada

   Article

   Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

   Insert

   cysteine-free SGFP2-N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S and C70M mutations relative to SGFP2

   Promoter

   CMV

   Available Since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Wnt6

    Plasmid

    #35913

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt6 (WNT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation G (567) --> A

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. BFP dest clone

    Plasmid

    #71825

    Purpose

    Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hAur-A D274A

    Plasmid

    #8511

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D274A, catalytically dead mutant

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-APPSwe-T2A-Hygro

    Plasmid

    #190805

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57 mini-ZsGreen

    Plasmid

    #222648

    Purpose

    Vector for cloning gene of interest for in vitro transcription

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    ZsGreen
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    mutated stop codon

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50466

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAureus-TnCAM

    Plasmid

    #199807

    Purpose

    vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

    Depositor

    Steve Salipante

    Article

    Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

    Insert

    transposon cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT07

    Plasmid

    #223379

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-puro GFP-p62 dC

    Plasmid

    #38282

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncate mutant 1-265 amino acids residues

    Promoter

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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