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pLKO.1_sh_ex15

Plasmid

#35166

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Rat Alpha2 delta-1 mutMIDAS pMT2

Plasmid

#58730

Purpose

expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

Depositor

Annette Dolphin

Article

Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

MIDAS site mutations (D259VSGS to AVAGA)

Promoter

SV40

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/BirA/FLAG-DDX54

Plasmid

#97063

Purpose

Expresses BirA/FLAG-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

BirA and FLAG

Expression

Mammalian

Promoter

tetO2

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-Actin-7

Plasmid

#55888

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Actin (ACTB Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-GP-2-CBX1 Chromo

Plasmid

#59697

Purpose

Contains histone modification interacting domain (CBX1 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX1 Chromo (CBX1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-MED31

Plasmid

#15427

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
TFORF3004

Plasmid

#144480

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PCGF2 (PCGF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TOX2

Plasmid

#192920

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TOX2 (TOX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pDN-T1GZmbh

Plasmid

#44522

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex13

Plasmid

#35165

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pDN-G1TCbt

Plasmid

#44515

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.2 (20-362)

Plasmid

#177845

Purpose

Bacterial Expression of SnRK2.2

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147768)

Plasmid

#76194

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDN-G1GZmbh

Plasmid

#44516

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 SHPmut

Plasmid

#113503

Purpose

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

F215A/E218A/QKL220-222AAA

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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