We narrowed to 15,981 results for: LEA

1. pSDMA67

   Plasmid

   #128356

   Purpose

   gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

   Insert

   Noureothricin (NAT)
   

   Use

   Unspecified

   Promoter

   ACT1

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. csr-1_son-1_mApple

   Plasmid

   #191741

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and mApple

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. csr-1_son-1_mCherry

   Plasmid

   #191742

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and mCherry

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNLS-LSSmKate2-N1

   Plasmid

   #31871

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIS.MTRIA-PRLH

   Plasmid

   #184618

   Purpose

   For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-PRLH
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. RECQL5 (RECQL5A-c104)

   Plasmid

   #98234

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RECQL5A (RECQL5 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EPHB3

   Plasmid

   #28165

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHB3 (EPHB3 Human)
   

   Tags

   His tag with TEV cleavage and Honeybee melittin S…

   Expression

   Bacterial

   Mutation

   Ligand binding domain (residues 39-211).

   Available Since

   March 18, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mEos4b-LaminA-C-18

   Plasmid

   #57511

   Purpose

   Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson, Loren Looger

   Insert

   LaminA (LMNA Human)
   

   Tags

   mEos4b

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. MTK4a_003

   Plasmid

   #123817

   Purpose

   Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NLS
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046422

    Plasmid

    #145515

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. HLTF

    Plasmid

    #28152

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    helicase-like transcription factor (HLTF Human)
    

    Tags

    thrombin cleavage

    Expression

    Bacterial

    Mutation

    Contains HIRAN domain (residues 51 to 171).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046173

    Plasmid

    #149126

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046397

    Plasmid

    #145479

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGC4780

    Plasmid

    #113408

    Purpose

    Translation reporter with inducible translation coupling (high native GFP expression)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    158_33121123_1
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046506

    Plasmid

    #145560

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046486

    Plasmid

    #145506

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4046351

    Plasmid

    #145449

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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