We narrowed to 1,399 results for: pBAD
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Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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MK01
Bacterial Strain#195090PurposeImproved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).DepositorBacterial ResistanceChloramphenicolAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
ExpressionBacterialPromoterpBADAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMS_375
Plasmid#182132Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMRDepositorInsertsretron RNA
ec.86 RT
CspRecT
Mutationincorporated rifampicin-resistance-conferring don…Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…PromoterpBADAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN-iRS3GG
Plasmid#98858PurposeExpresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertPheS cassette to be selectively replaced by unique asRNA probes
ExpressionBacterialPromoterpBADAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N748D
Plasmid#60726PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEL3016
Plasmid#232528PurposeExpression plasmid of LetA(T402A) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA T402APromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2913
Plasmid#232523PurposeExpression plasmid of LetA(ΔZnR-N) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA ΔZnR-NPromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2914
Plasmid#232524PurposeExpression plasmid of LetA(ΔZnR-C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA ΔZnR-CPromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2921
Plasmid#232525PurposeExpression plasmid of LetA(S364A) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA S364APromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL3014
Plasmid#232526PurposeExpression plasmid of LetA(D181A) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA D181APromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL3015
Plasmid#232527PurposeExpression plasmid of LetA(S321A) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA S321APromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2638
Plasmid#232501PurposeExpression plasmid of of LetA with LetB(E854B)DepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetB E854AmberPromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2745
Plasmid#232504PurposeExpression plasmid of of LetA(W162B) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutationLetA W162AmberPromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only