We narrowed to 2,563 results for: Endog

1. pLV.U6gLacZ.EFS-NS.H2B-RFP

   Plasmid

   #170363

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS-NS

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

   Plasmid

   #104462

   Purpose

   Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

   Insert

   mCherry-subT391A-FHA1-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS1623

   Plasmid

   #63795

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   tDimer2-Twin-Strep tag followed by HIS5
   

   Use

   E. coli cloning vector

   Tags

   tDimer2-Twin-Strep

   Expression

   Mutation

   Promoter

   none

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS1322

   Plasmid

   #63792

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eCFP-Twin-tag followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   eCFP-Twin-tag

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS1112

   Plasmid

   #63784

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eSapphire-Strep-tag II followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   Sapphire-Strep-tag II

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGS1522

   Plasmid

   #63794

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eVenus-Twin-Strep tag followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   eVenus-Twin-Strep

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS1422

   Plasmid

   #63793

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eCitrine-Twin-tag followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   eCitrine-Twin-tag

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGS1512

   Plasmid

   #63788

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eVenus-Strep-tag II followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   eVenus-Strep-tag II

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGS1312

   Plasmid

   #63786

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eCFP-Strep-tag II followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   eCFP-Strep-tag II

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_GPHN.FingR-mKate2-IL2RGTC

    Plasmid

    #46297

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM4787

    Plasmid

    #120088

    Purpose

    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-Amp_CRISPIE donor B5

    Plasmid

    #172846

    Purpose

    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_VCL sgRNA / hSpCas9

    Plasmid

    #172837

    Purpose

    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid

    #172836

    Purpose

    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_CALR sgRNA / hSpCas9

    Plasmid

    #172838

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    F9 promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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