We narrowed to 4,428 results for: Erf

1. pCellFree_G03 WAVE 2

   Plasmid

   #67098

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WAVE 2 (WASF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbdCas9S_P0526-sgRNAmreB

   Plasmid

   #149654

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, P0526

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-3xhfYFP

   Plasmid

   #186544

   Purpose

   Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP (3X)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRIME-CMV-LNGFR-recipient

   Plasmid

   #11660

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   LNGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC18-H1 RNAi

   Plasmid

   #87355

   Purpose

   RNAi expression vector based on H1 promoter and pUC18 vector backbone

   Depositor

   Saroj Mathupala

   Article

   Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

   Insert

   H1 RNA Promoter from U87MG glioma cells
   

   Use

   RNAi; Shrna expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 RNA

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-myc-hIFITM3

   Plasmid

   #58461

   Purpose

   Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CROP_C9_Puro_DNMT3B

   Plasmid

   #183284

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   DNMT3B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS NL4-3 envFS eGFP IN-D116A

   Plasmid

   #101333

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Integrase D116A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ER2-hfYFP

   Plasmid

   #186518

   Purpose

   Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNL4-3 envFS eGFP NoStarts

    Plasmid

    #101342

    Purpose

    All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No methonines in Gag

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK010

    Plasmid

    #69874

    Purpose

    expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

    Depositor

    Robert Kittel, Georg Nagel

    Article

    Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

    Insert

    Channelrhodopsin-2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D156C (XXL)

    Promoter

    Drosophila Hsp70

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROP_C9_Puro_DNMT3A

    Plasmid

    #183283

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Plasmid

    #192123

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. InterCatch-1xL

    Plasmid

    #186907

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-IREShygro2

    Plasmid

    #153966

    Purpose

    Expresses EGFP in mammalian cells

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    EGFP
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CMV promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROP_C9_Puro_PARP1

    Plasmid

    #183312

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PARP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Enzymatically dead Cas9

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_TmiR_Luc-A

    Plasmid

    #25760

    Purpose

    Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge